Therapeutic RNA?

Short interfering RNA (siRNA): tool or therapeutic?

Gene silencing by siRNA (short interfering RNA) is a still developing field in biology and has evolved as a novel post-transcriptional gene silencing strategy with therapeutic potential. With siRNAs, virtually every gene in the human genome contributing to a disease becomes amenable to regulation, thus opening unprecedented opportunities for drug discovery. Besides the well-established role for...

The therapeutic potential of RNA interference.

In recent years, we have witnessed the discovery of a new mechanism of gene regulation called RNA interference (RNAi), which has revitalized interest in the development of nucleic acid-based technologies for therapeutic gene suppression. This review focuses on the potential therapeutic use of RNAi, discussing the theoretical advantages of RNAi-based therapeutics over previous technologies as we...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...

Exploring the Immunogenicity and Therapeutic Applications of Boranophosphate - modified RNA : siRNA and RNA Aptamers

Exploring the Immunogenicity and Therapeutic Applications of Boranophosphatemodified RNA: siRNA and RNA Aptamers by Mariam Lucila Sharaf Department of Chemistry Duke University Date:_______________________ Approved: ___________________________ Barbara Ramsay Shaw, Supervisor ___________________________ Katherine J. Franz ___________________________ Thom LaBean ___________________________ Bruce ...